The murine toxin of Pasteurella pestis: a study in its development.

I NTRODUCTION................................................................ 177 ISOLATION AND PURIFICATION OF THETON .....................................T 178 IMMUNOLOGICAL PROPERTES .................................................... 179 MECHANIM OF ACTION OF THE ToXIN .......................................... 180 Effect of the Toxin on Microbial Extracts and Crude Tissue Homogenates........... 180 Action of the Toxin on Mammalian Mitochondria ......... ....................... 180 Effect on respiration....................................................... 180 Ability of the toxin to induce mitochondrial swelling............................ 182 Effect on the electron transport system....................................... 183 Effect on mitochondrial ion accumulation...................................... 185 DISTRIBUTION AND SYNTHSIS OF PLAGUE MuRm ToxIN.......................... 185 Location of the Toxin in the P. pestis Cell...................................... 185 Resolution and Isolation of Two Toxic Components............................... 186 Selective Inhibition of Murine Toxin Synthesis by Tryptophan Analogues............ 186 Antagonism ofanalogue bacteriostatic action and the selective inhibition of toxin... . 187 Toxin protein content in extracts from analogue-treated cells..................... 187 Inhibitory effect of analogues on spheroplast toxin content...................... 188 SummARY.................................................................... 188 LrrERATURE Crnu. ............................................................ 188